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To assess in a parallel assay the correlation between lethal potency and inhibition of system xc− activity, we examined glutamate release using a high throughput, 96-well Amplex Red assay system in human CCF-STTG1 astrocytoma cells.
The number of samples is too small to draw definitive conclusions, but these results suggest that if the accuracy of RNA estimations is increased (based on the correlation with the RT-PCR assay), the correlation between RNA and protein levels is more likely to be high (Additional file 9).
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We further assayed the correlation between apoC-III ELISA and 9443 MALDI results per each sample; ELISA and MALDI results showed significant correlation (p<0.0001, Kendall's ran correlation tau).
We first assayed the correlation between gene signals of the two duplicates: the correlation is very good for high values of rpbp, and progressively decreases as the rpbp decreases.
These assays measure the correlation between increased enzymatic activities towards model substrates in resistant mosquitoes and are a general indicator of the enzyme families putatively involved.
From the MN assay comparison performed here, we anticipate that, as laboratories gain experience in both assays, the correlation between the titers obtained for the 2-day ELISA and 3-day HA MN assays will improve.
We observed the same pattern within the 20°C live E. coli assay where the correlation between these two traits declined monotonically from +0.535 to −0.030 with increasing PQ level (Additional file 1: Table S4).
However, access to nanodrop spectrophotometers is limited at some institutions making it difficult to perform the assay, and the correlation between nanodrop and the traditional agar plate analysis is not ideal (i.e., r = 0.458), limiting its utility as a proxy for actual bacterial killing (Liebl and Martin, 2009).
Global concordance for each culture/tissue pair was assayed evaluating the correlations between whole-genome CN profiles and SNP allelic calls.
Thus, we next performed a thermal-shift assay to investigate the correlation between enzymatic activity and thermal stability of PKM2 proteins.
To evaluate oxidative stress indices in follicular fluid (FF) by a novel thermochemiluminescence (TCL) assay and investigate the correlation between TCL and IVF cycle parameters.
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