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The cells were resuspended in medium, and an aliquot was collected in order to assay the amount of internalized radioactivity using the automated gamma counter.
We initially performed ICC against laminin to assay the amount of laminin on the microposts.
Prior to assay, the amount of enzyme and digestion time was optimized to obtain above 90% purity of mononucleosomes.
We cued D1R WT and KO mice on an AL diet to assay the amount of activity that occurs as the result of a disturbance independent of FAA.
Following centrifugation at 21,880 × g for 5 min, Glucose UV reagent (Raichem, USA) was added to assay the amount of biological index glucose contained in the serum.
To assay the amount of attraction that pollen tubes had toward an ovule, we calculated the fraction of pollen tube tips that were within a certain distance of a micropyle that grew either closer to (f closer ) or farther from (f farther ) that micropyle by the next time point (Δ t = 20 min).
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These relationships were identified for assaying the amount of CEAs.
Nanoparticles were tested for in vitro cytotoxicity by conducting a LIVE/DEAD assay, and assaying the amount of ROS generated by the particles.
When cell-free aliquots of the medium from cultures were assayed, the amount of LDH activity could be used as an indicator of membrane integrity.
Furthermore, the relationship between ΔM S/M S and Φ CEA could be described by a characteristic logistic function, which was appropriate for assaying the amount of CEAs.
The binding and inactivation steps of this reaction are investigated by incubating the enzyme with inhibitor and assaying the amount of activity remaining over time.
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