Exact(3)
We generated a null mutation in pacman (pcm ) and confirmed, using a novel assay, that it has no detectable 5′-3′ exoribonucleactivityviny in vivo.
When analyzing diversity in the cultivated germplasm, we observed that the cultivar Orbois was also completely homozygous for all fragments re-sequenced at the top of chromosome 18, and confirmed by qPCR assay that it was also due to real homozygosity (data not shown).
However, Ohno and First [ 28] pinpointed the limitations of the Folin-Ciocalteu assay that it is suited only for samples extracted by water, and interference by organic matter, such as sugars and aromatic amines, makes it impossible to precisely measure the amount of phenolic acids in citrate-extracted soils.
Similar(57)
We used a sensitive PCT assay that made it possible also to determine PCT levels between 0.06 ng/ml and 0.5 ng/ml.
We focus on one such compound, BRD6897, and demonstrate through secondary assays that it increases the cellular content of mitochondria as evidenced by fluorescence microscopy, mitochondrial protein content, and respiration, even after rigorous correction for cell size, cell volume, or total protein content.
Dennis et al. further characterized the 5′ upstream of KIAA0319 and identified a SNP marker rs9461045 that was not only significantly associated with DD and previously reported to be associated with reading-related traits but it was also found by luciferase-based assays that it could influence gene expression, possibly by alteration of the binding site to transcriptional silencer OCT-1 [ 10].
But he advises caution: a polyclonal antibody is "usually raised only against the peptide of limited size, and therefore may in fact act equivalently to monoclonal in ChIP assays". That's when it helps to know the immunizing peptide to confirm coverage of the protein target's distinctive part.
The newer assays that have replaced it have lower limits of detection below 50 copies/ml.
However, one of the limitations of the biofilm microtiter assay is that it is an endpoint assay and provides no information about biofilm developmental stages such as those that can be analyzed in flow cells.
One major advantage of the FRET assay over the SDS-PAGE-based assay is that it can be easily configured to a high-throughput format for analysis or for screening.
While tyramine is a major neurotransmitter in insects, its low potency in our assay suggests that it might not serve as an endogenous OR modulator.
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