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For the remaining 38 variants (in 18 other genes), we designed a custom iPlex panel in a 2-well assay (Table 1).
Real-time qPCR was performed to determine and calculate the FAIRE enrichment with same primers used in ChIP assay (Table 1).
Similar data were obtained by the TBARS assay (Table 1).
Voriconazole concentrations were measured by using a validated high-performance liquid chromatographic assay (Table 51).
This sample had the lowest copy number using ND5 assay (Table 3).
Effects of those mutations were tested with the CPM assay and the affinity MS assay (Table 2).
We compared functional responses of all three ligands in the [35S]GTPγS binding assay (Table 1).
However, their antimicrobial activities were very similar by MICs assay (Table 1).
The xthA/nfo mutant was 4-fold attenuated in an i.p. competition assay (Table 4).
The lexA3 strain was fully virulent in a competition assay (Table 5).
The sodC mutant and wild type strain competed evenly in this assay (Table 3).
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