The phrase "assay synchronized" is not standard in written English and may cause confusion.
It could be used in a scientific or technical context where "assay" refers to a procedure to measure the presence or amount of a substance, and "synchronized" implies that multiple assays are conducted simultaneously or in a coordinated manner.
Example: "The laboratory conducted the experiments in an assay synchronized manner to ensure consistent results across all samples."
Alternatives: "assay coordinated" or "assay aligned".
In the first assay, synchronized L1 stage worms were initiated on bacterial feeding, and were examined under Nomarski optics at 48 hours for subsequent attainment of larval stages.
For both types of assay, synchronized unstarved adult animals were rinsed off culture plates with S basal for odorant assays and sterile ddH2O for water soluble chemotaxis assays.
For the survival assay, synchronized L4 worms were suspended in a medium containing 95% M9 buffer, 5% BHI and 10 µg/ml cholesterol (Sigma-Aldrich).
In this assay, synchronized, mature, blood-stage parasites were allowed to invade fresh erythrocytes and parasitemia was measured before and after invasion by flow cytometry (Fig. 1).
For this assay, synchronized L1 stage worms were fed on hlyA intact (E7946 and CVD109) and hlyA deficient (CVD110 and HNC45) strains of V. cholerae for 72 hours, and the optical density of the worms were evaluated by EXT measurements (Methods).
Therefore, in this first assay, synchronized adults were fed with Paraquat (1,1′-dimethyl-4,4′-bipyridylium 1,1′-dimethyl-4,4′-bipyridylium 1,1′-dimethyl-4,4′-bipyridylium 1,1′-dimethyl-4,4′-bipyridylium cells andichloriderenewandindependent of the late response involving increased ISC proliferation.
For modified growth inhibition assays, synchronized cultures were incubated for 24 h before addition of the drug to allow for the appearance of late forms presenting the epitope recognized by BM1234 bound to immunoliposomes.
For all assays synchronized L1 larvae were grown on the carminc acid NGM plates and assayed as one-day old adults.
For lifespan assays, synchronized populations were collected by allowing gravid adult hermaphrodites to deposit eggs onto NGM plates containing OP50 bacteria or indicated RNAi bacteria.
For regular thermotolerance assays, synchronized young adult animals (72 h post hatching) were grown at 20°C or 25°C and then transferred to 35°C and checked every hour for death animals.
Since the fer-1 alleles are temperature sensitive, animals used for assays were synchronized by the hypochlorite method then grown at the restrictive temperature of 25°C to generate staged young adults.
