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The nine cases of cluster compression detected in our assay support this hypothesis.

Both the GTP hydrolysis assay and the GTP exchange assay support the notion that ARHGEF7 might be the first identified GEF for LRRK2.

The authors would like to thank Hillary Conway and Norman Jones for assay support, Angela Freels for maintaining N. benthamiana, and Andrew Marsh for editorial assistance.

The results of the reporter gene assay support the prediction from in silico analysis; the haplotypes C-D-C-C and T-D-C-T had significant lower activity than the C-I-T-T.

The results of the challenge assay support the previous reporter knockdown data that lhRNA knockdown efficacy is primarily mediated by first position siRNA sequences located at the base of the duplex stem.

These results, consistent with those from the availability assay, support the finding that Lfng inhibits Jag1-Notch1, but not Dll1-Notch1, cis-inhibition.

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Similar(47)

Finally, radial diffusion assay supported that these peptides displayed functional antimicrobial activity against E. coli and Staphylococcus aureus standard strains.

The fact that luciferase from as little as 50 cells could be readily detected by our assay supports this assumption.

Significant effects on Listeria monocytogenes were also observed with Nalp3 and TLR2 LRR domains using an ATP-based assay supporting this conclusion (Figure S4).

Of the 79 interactions identified, 11 of them were reciprocal interactions that were tested and confirmed in both orientations in the YTH assay (Supporting Information Table S2, Figures 2 3), strongly supporting the validity of these interactions.

The enzyme activity assay supported this result (Fig. S6, Supporting information).

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