Sentence examples for assay successful from inspiring English sources
With this assay, successful amplification of the five markers was obtained in 90% of cases regardless of their origin from tumour or normal matching DNA.
They state that to deem an assay successful it must possess three distinct clusters: the water controls must be negative, the number of genotypes callable must be >90% and the minor allele frequency should be >2%.
The overall success of SNPs development in Acacia hybrid will depend on short read sequence quality, a highly robust SNP detection approach to identify sequencing error and with enough sensitivity to detect rare SNPs to increase assay successful rate, appropriate assay design and genotype calling approach to obtain high quality genotypes.
We found that the design score of failed assays and successful assays were not significantly different using t-test (p≤0.05).
Out of 96 SNPs, 86 assays were successful, giving an assay success rate of 89.6%.
The assay success rate was calculated based on the number of successful assays divided by total number of assays while the conversion rate was calculated based on number of polymorphic SNPs divided by total number of assays.
As with other currently used mtDNA typing methods such as sequencing analysis of multiplexed short PCR products, hybridization probe based assays, or minisequencing, successful assay designs might be challenging or even impossible for particular sites in such "unstable" sequence environments.
To confirm that the assay was successful, positive controls of WSX1-MUT ran side by side and in the same gel with full-length WSX1 are included in Figure S1.
The Taqman assay was successful in detecting the expression of all miRNAs with ASRP inflorescence expression data.
In each assay, the successful was determined by calculating the percentage of reduction: (2) % Reduction = [ 1 − Parasitic stage s day 0 Parasitic stage s day 30 ] × 100.
