Exact(35)
The plasmids were generated by homologous recombination directly in the S. cerevisiae assay strain YSH1770, silenced for endogenous aquaporins AQY1 and AQY2 (10560-6B MATa leu2::hisG trp1::hisG his3::hisG ura35-2 aqy2D::KanMX 18,25::KanMX 18,25.
For pentachlorophenol, the E. coli K12 assay strain was engineered to produce a PCP inactivating enzyme pentachlorophenol-4-monooxygenase (PcpB).
In the case of erythromycin, the E. coli K12 assay strain was engineered to produce an erythromycin inactivating esterase, PlpA.
In a mating assay, strain K76A successfully mated with HR42-11T (α-type) and K76AL mated with MCF4741 (a-type) (Fig. 4).
In the capillary assay, strain CNP-8 exhibited chemotaxis toward 2C4NP with a maximum chemotaxis index of 37.5 when the concentration of 2C4NP was 0.5 mM.
The resulting fragment was transformed into the CEN-proximal cohesion assay strain.
Similar(25)
The malacidins were screened against a panel of assay strains and pathogenic bacteria as indicated in Supplementary Table 3. MIC assays were performed in duplicate in 96-well microtiter plates on the basis of the protocol recommended by the Clinical and Laboratory Standards Institute29.
For drop plate assay, strains SJ98 was grown in MSM + LB (v/v = 4 1) to exponential phase, and then induced by 0.3 mM of target substrate (2C4NP, 2C5NP, PNP or MNP) for 8 h.
In the qns1 bioassay assay, strains tested for NR export are grown overnight in SDC.
As in the antifungal assay, strains with similar secondary metabolites displayed similar, but never identical, inhibition profiles (Fig. 3).
To construct CEN-proximal cohesion assay strains containing ctf7eco1-1 ADE ctf7eco1-1 ADEining wild typlasmid was geneticontainingneered to contain ctf7eco1-1 DNA that harbors G211D.
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