Fig. 1 Badapple score dependence on assay-active and assay-tested statistics.
The assay performance statistics generated from screening plates were well within acceptable screening parameters (Z′ 0.72 ± 0.04) and the replicate potency determinations correlated well, yielding errors below 45% for all bar one compound (Table 3).
For longevity and oxidative stress assays, Kaplan Meier statistics were used to determine the median lifespan/survival period, and log rank test was used to calculate P value to determine statistical significance.
It consisted of eight reporting domains: (1) goal and hypothesis; (2) subject eligibility; (3) specimen characteristics; (4) assay protocol; (5) statistics; (6) subject flow; (7) results; (8) discussion.
Although this polarization effect remains to be quantified (dependence on time of culture, on levels of cell to cell competing condition, etc)., these preliminary assays provide clear statistics on hMSC proliferation at comparable rates with respect to the culture on standard substrates.
See the Extended Experimental Procedures for details of cell culture, antibodies and reagents, autophagy and endocytosis assays, microscopy, and statistics.
These results include computed Badapple scores, plus assay, substance and sample statistics for each scaffold.
Considering the heterogeneity of cancer, this number is far too low to validate any assay by conventional correlative statistics.
The assay performance and plate statistics (sampled from 26 plates; 384-well format) are clearly acceptable for a cell-based assay (S/B of 30; S/N of 11; Z′ of 0.67) [ 23].
MKG helped to carry out the animal experiments, performed the immunohistochemistry, enzyme-linked immunosorbent assay, multiplex analysis, and statistics, and helped to perform the morphometric analysis, to analyze the results, and to write the paper.
