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Resurrecting 'good' drugs prematurely culled at the hERG assay stage.
KG supported and commented the mechanical design of the assay stage and provided critical intellectual input.
WH built the involuntary assay stage, conducted the involuntary contraction assay, and revised the manuscript.
RA involved in the assay stage mechanical and electrical design and involuntary contraction assay data acquisition and analysis.
This assay subjected each sample to both an empty site assay (stage 1) and an internal primer test (stage 2).
The involuntary contraction assay stage was built using building blocks from a construction set (Fischertechnik ®, Germany) on top of a base plate.
Similar(52)
This suggests that the use of a better extraction instrument would increase the proportion of lytA-positive samples with low Ct-values and consequently increase their likelihood to be correctly serotyped using the serotyping assay (stage-1 analysis).
Electrophoresis controls all assay stages, affording purely electronic operation with no pumps or valves needed for fluid control.
In fact, we found that initiating an acute atrazine exposure at the end of the 96-hr FETAX protocol (~ NF < stage 44) and just before the start of the XEMA assay stages (NF stage 52 and later) resulted in severely abnormal organ phenotypes and increased apoptosis.
In order to integrate the assay stages and obtain high immobilization efficiencies, the μWestern makes dual use of the separation axis: the axis forms the molecular sieving dimension during PAGE and also forms the path for antibody probe introduction during probing (i.e., antibody is introduced through the protein separation gel).
Kübler-Ross's five-stage topography of grief ("Denial"), and even a few who are tentatively assaying Stage 3 ("Bargaining"), are clinging to.
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CEO of Professional Science Editing for Scientists @ prosciediting.com