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A custom TaqMan® assay spanning the junction between exons 14 and 15 of rhesus Huntingtin was used to measure Huntingtin expression.
Included in the validation process, was a comparison of these real-time PCR assays and newly developed conventional PCR assays targeting much larger areas of the 102-kb region (including one assay spanning hmsR and hmsF, one spanning hmsH and hsmF, one targeting hmsF, and one targeting irp2).
Data is displayed as mean endpoint calcium response (Figure 4B) as well as a continuous assay spanning 30 s (Figure 4C).
In the case of T7, the initial fitness W0 was based on an assay spanning six generations, so the effect is possibly substantial.
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The assay spanned multiple CpG sites, including the specific CpG interrogated on the Infinium HumanMethylation450 BeadChip array.
Our assay spanned six CpG sites, including the specific CpG interrogated on the Illumina 27 K array.
The metal concentrations used in this assay spanned a range from moderately to severally toxic, with the highest concentrations of each metal causing complete loss of turger in the seedlings.
Thus, the qualified range of the miR-34a assay spanned 6 orders of magnitude, ranging from 2.5 × 10 7 to 2.5 × 10 1 ng miR-Rx34, which equals an estimated blood concentration of 6.2 × 10 5 6.2 × 10 ng/mL.
The primers for each assay spanned the proximal BRCA1 promoter region and were carefully designed so that the methylation status of the highly homologous pseudo BRCA1 region [ 22] was not assessed.
As the standard fitness assay spans several generations and thus might have partitioned some of the nonlethal component into W 0 e − U L, the T7 study estimated initial fitness in two ways.
To study this hypothesis further, the prediction network method should be repeated with a dense matrix of assays spanning diverse experimental conditions and compounds tested against all assays.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com