Exact(14)
For neuropathological assay, slides of brains were analyzed by HE staining.
B. pseudomallei strain NCTC 10276 was used to prepare immunofluorescence assay slides.
For immunocytochemistry analysis, assay slides were prepared (described in detail elsewhere [ 7]).
MM imaged the migration assay slides, counted cells, and quantified the results.
LCLs were re-suspended in 1% low-melting-point agarose (Sigma-Aldrich) and plated on 20-well comet assay slides.
Cells were re-suspended in 10% low-melting-point agarose and immediately pipette 50 μl onto two-well comet assay slides.
Similar(46)
IS-RT-PCR was performed on consecutive sections for each xenograft: section A) RNase/DNase negative control; B) β-actin positive control; C) MT1-MMP assay slide; D) MMP-1 assay slide; and E) MMP-3 assay slide.
The cell suspension was spread onto a standard comet assay slide (Singh et al, 1988) and lysed in a neutral lysis buffer containing 0.1% LiDS, pH 8.0, and 0.03 mg ml−1 proteinase K, at 37°C overnight followed by alkaline lysis, pH 10 for 1 h at 4°C.
For the recombinant protein inhibition assays, slides were preincubated with the indicated quantities diluted in cytoadhesion medium for 1 h at 37°C.
For binding assays, slides were initially thawed and then fixed in 0.1% paraformaldehyde.
For FISH assays, slides were previously coated with 3-aminopropyltriethoxysilane (APES; Sigma-Aldrich, Dorset, UK) and were always freshly prepared.
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