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(A) GST-pull down assay showing the interaction between NS1 and Rac1.
m, Representative comet assay showing the tail moment of parental and cGAS knockout HCA2-H15c cells under alkaline conditions.
We identified neutrophil-attracting chemokine IL-8 released by infected mast cells and translated the findings into a functional assay showing that human neutrophils migrate to infection supernatants.
a, Results of the MTT assay showing the proliferation of PC-9 cells that had been stably transfected with pcDNA3.1-HA (HA) or HA cGAS (HA cGAS).
(d) Immunofluorescence assay showing the distribution of WT Gag or CASP3*-Gag (CASP3*) in 293T cells transfected with either WT or CASP3*-gag-pol (CASP3*) expression vector.
The antioxidant activity was measured by DPPH assay, showing that the strongest antiradical activity was detected when the 3,4-DHPAA concentration was higher during electrolysis experiments.
MTT assay showing significant inhibition against HepG2 and MCF-7 cells for the DOX/GNPs-loaded micelles.
p, Representative results of the comet assay showing the tail moment of skin fibroblast cells isolated from the tail of wild-type and cGAS knockout mouse under alkaline conditions.
The ability of Ca2+ to revert AMPylation supported by Mg2+ was further confirmed in a kinetics assay, showing that addition of Ca2+ suffices to switch EfFIC from the AMPylation regime to the deAMPylation regime (Fig. 3e).
We then evaluated species prediction on the union of data sets MTBC_A2 (1157 MTBC clinical isolates, Supplementary Data 7) and Myco_Retro (147 Mycobacterial isolates, Supplementary Data 10), where species had been identified by Hain assay, showing results in Fig. 5a.
c, Neutralization assay showing that ADI-15946 has enhanced neutralization of a GP construct lacking the β17 β18 loop compared to wild type (WT), probably due to increased access to the 310 pocket.
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