The phrase "assay setting" is correct and usable in written English.
It can be used in contexts related to scientific or laboratory environments, particularly when discussing the conditions or parameters under which an assay is conducted.
Example: "The assay setting must be carefully controlled to ensure accurate and reliable results."
Alternatives: "test environment" or "experimental conditions".
Second, a promoter of a target gene with a TFBS deleted can be compared to a wild-type promoter to see if the TFBS confers any biological activity in a promoter-reporter assay setting.
Further, the role of solubility as a governing factor for colloidal aggregation formation within a specified assay setting is examined and considered as a possible cause of a high cross-assay activity score.
39 Based on these considerations we decided to choose compounds that are at the upper end of the fragment space or slightly bigger and more complex (Table 1) with the goal of obtaining initial hits with affinities that are detectable in a biochemical assay setting but are still amenable to optimisation.
However, the two assay sets have very similar averages and standard deviations to each other, and hence the analysis and discussion below, which are based on the 1,528 assay set, also hold for the 843 assay set.
The results from the 843 assay set are included in Additional file 4. The similarity score distributions from the two assay sets have very similar averages and standard deviations to each other, and hence, the conclusions drawn from the 1,528 assay set should also be valid for the 843 assay set.
Therefore, a subset of the 1,528 assays were generated by selecting 843 assays which have active compounds only (without any inconclusive or unspecified compounds), and the same analyses were performed both for the 1,528 assay set and the 843 assay set.
Three endogenous controls were added to the assay set.
On the day of assay set up, cell count and viability were determined using a Guava Cell Counter.
PCR ramping protocols were standardised for all QuantiTect primer assay sets at 50°C for 2 min, 94°C for 5 min, followed by 40 cycles of 30 s at 94°C, 45 s at 55°C and 45 s at 72°C.
Alternatively, the assay set up may be suppressing courtship.
Genes in the current assay set are linked to various toxicity end-point classes.
