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On the day of assay set up, cell count and viability were determined using a Guava Cell Counter.
A comparable inhibition occurred in the HALO-Hemogenix luminescence assay, set up with different growth factor cocktails to reveal proliferation of progenitors with distinct differentiation potentials (CFU-C, CFU-C+BFU-E and CFU-C+BFU-E+CFU-GEMM) (Fig. 2C).
Alternatively, the assay set up may be suppressing courtship.
Due to our assay set up, some mutations might affect other processes besides conjugation per se.
For each assay set up a control universal methylated DNA standard (Zymo Research; Cambridge Biosciences) was also taken through the process to ensure that bisulphite conversion was complete.
In addition, recent reports show that even rare mutations in codon 600 can be detected using pyrosequencing with a customer designed assay set up [ 24, 33].
Similar(50)
TaqMan assays set up for four of these sequences confirmed the relative expression levels (Table 4).
The background of the described assay was set up with sera of elephants, which were consistently found on the non-detectable range of the assay, but still ~0.1 OD higher than naïve rabbit serum.
Each assay was set up at least in duplicate.
The assay was set up in the Light Cycler instrument using the Fluorescence Resonance Energy Transfer platform.
The winter assay was set up in a field and cultivated with staked tomatoes cv.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com