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Discover LudwigThe phrase "assay section" is correct and usable in written English.
It can be used in contexts related to scientific or laboratory reports, particularly when referring to a specific part of a document that discusses assays or testing procedures.
Example: "In the assay section of the report, we detail the methods used to analyze the samples."
Alternatives: "testing segment" or "analysis portion".
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Statistically significant differences in cell growth assays: Two-tailed t tests were performed to calculate p values on Graphpad Prism Version 7. Number of replicates are provided above, in the Cell growth assay section.
The methodology of the assay is described in enzyme assay section.
Enzyme activity was then assessed spectrophotometrically as described below in the "Enzyme assay" section.
The cell treatments were the same with "Detection of apoptosis by annexin V assay" section.
After incubation for 48 h, fluorescence quantitative analysis was carried out as described in the competition assay section.
Strains CEN.PK 113-5D, 5Dgpp2∆, 5Dgpp2∆ and 5Dgpp1∆gpp2∆ were cultivated, cells harvested and cell free extract prepared as indicated in phosphoketolase assay section above.
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For immunohistochemical assay, sections were made from trigeminal nucleus caudalis (TNC) tissues from different groups and incubated at 60 °C overnight before dewaxed with dimethylbenzene.
For the simultaneous in situ detection of apoptotic cells (TUNEL assay), sections were first processed as above and after color development with DAB, they were incubated with terminal deoxynucleotidyl transferase (TdT) (Roche Diagnostics, Mannheim, Germany) at 37oC for 1 h in a humidified chamber.
For the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, sections of liver (3 μm in thickness) were stained with the ApopTag Apoptosis Detection Kit (Merck Millipore, Billerica, MA, USA) as described in the manufacturer's instructions.
The treated cells (as described in "Caspase assays" section) were fixed with 4%% paraformaldehyde for 10 min in situ and then stained with Hoechst 33258 (5 μg/mL) for 3 min at room temperature in the dark.
For that, the same experimental procedure described for the colonization assays (section 2.3) was used, except that, before transferring to eppendorfs for sonication, each coupon was gently immersed in ultrapure sterile water and then tilted to allow the liquid to flow over the surface.
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