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These results were demonstrated by ectopic expression into highly metastatic cells in an experimental in vivo assay (reviewed in [1]).
In the 'minisequencing' assay (reviewed in [ 48]), target hybridisation is combined with an enzymatic primer extension assay.
Currently, most genotyping methods require that the target region of the genomic DNA be amplified using PCR prior to the genotyping assay (reviewed in [ 1, 2]).
Researchers have used the growth response of S. faecium to measure the amount of non-methyl-folate in biological extracts and the amount of 5-methyl-THF, which they calculated by subtracting the S. fecium value from the value they obtained for total folate with the use of the L. casei assay (reviewed in reference 9).
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Importantly, these three genes can counteract the effects of interferon α in cell-based assays (reviewed in [10]).
A variety of methods for lineage analysis, generally termed clonal assays (reviewed in [5]), rely on marking some cells and tracing their progeny.
Of the approximately 500 assays reviewed, about two dozen assays were selected as high priority.
The existence of a hemifused state is supported by fluorescence and electrophysiology assays (reviewed in Chernomordik and Kozlov, 2003, 2005).
Out of the 18 assays reviewed, the top three of the selected assays focused on TH disruption via transport protein binding [ 3].
Studies with DNA damaging agents are used to gain insight in how particular lesions behave in different assays (reviewed in Kupiec 2000).
Although the involvement of Oct-1/2 in immunoglobulin gene expression and B-cell development could not be confirmed in knock-out mice [ 31, 32], a large number of immunomodulatory and inflammatory genes have been shown to be targets of Oct-1 in vitro and in cell-based assays (reviewed by [ 33, 34]).
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