For rFVIIIFc, individual one-stage to chromogenic assay ratios ranged from 0.6 to 2.6 (Table 3).
In this previous study, which was based on raw order volumes only, the functional assay to antigen assay ratios were 0.53, 0.54 and 2.59 for protein C, protein S and antithrombin, respectively.
In in vitro assays, ratios of Tregs to target cells that are between 1∶1 to 1∶4 are typically used for suppression of T cell activation [1], [6], [7], [42].
In this assay, the ratio of the two RNA populations at a given array element reflects the enrichment of the respective mRNA by the PUM affinity purification [35], [36].
The assays we developed – an antibody-based assay (1B3/9B9 ratio) and a restriction analysis of the PCR product – distinguish easily between elevation of blood ACE due to granulomatous diseases (sarcoidosis in particular) or Gaucher disease and hyper-ACE-emia due to lack of transmembrane domain, thus preventing false diagnosis and unnecessary treatment.
The protocol for the assay for ratio of E2 to E6/7 was modified to include dual standard curves of E2 and E6/7.
Only 6 of the 65 genes that were considered toxic on solid medium did not meet the cutoff that we used to identify a toxic gene in our pooled liquid growth assay (Log2 ratio < −1).
For rescue assays, the ratio of unfertilized oocytes to fertilized eggs was calculated daily.
For the second assay, the ratios 2AA:1BB; 1and1AA; 1AA:2BB; and 1AA:3BB were prepared to simulate AAB, AB, ABB and ABBB genotypes, respectively.
P19 cells were assayed for ratio of reduced to oxidized glutathione using the GSH/GSSG-glo kit (Promega) according to the manufacturer's instructions.
