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As the assay progressed over the following days, the number of viable cells increased on all substrates.
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Infection status of zebrafish was confirmed through PCR and colonization assay as time progressed and lesion development in the tails of zebrafish was also examined.
Specifically, Börjesson et al. (2009) showed that the concentration of MRSA as measured by real-time PCR assays decreased as treatment progressed from approximately 6 × 10 to 5 × 10 mecA genes per 100 mL from inlet to outlet, except for a peak in activated sludge reactor samples of 5 × 10 mecA genes per 100 mL (Börjesson et al. 2009).
The work progressed to behavioural assays.
In light of well publicised withdrawals of compounds from the US market, 1 drugs with high risk of QT prolongation in such assays may simply not be progressed into human trials.
Lineage-marker-negative (Lin-) BM was used as a starting population for in vitro erythropoietic MN experiments, and terminal erythropoiesis progressed normally as assayed by flow cytometry and histological examination (data not shown).
In parallel with the droplet-based assay, a progress curve for the reaction carried out under the same conditions, but without encapsulation, was recorded in a microplate.
However, this pattern was reversed as evolution progressed: the last two assay points showed significant differences (PE > DE, Fig. 3).
To measure the initial (and maximal) rate, enzyme assays are typically carried out while the reaction has progressed only a few percent towards total completion.
As the understanding of autophagy has progressed, so have the available techniques and autophagy assays [84].
OsINV3 promoter:GUS assay for panicles at progressing stages of panicle development were studied to isolate the temporal and spatial expression pattern in the panicles.
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