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The IAC assay probe was labelled with TYE665 and Iowa Black® RQ-Sp (IBRQSp).
The anti-GST 5′ and 3′ SH2-PLA probes were diluted in Assay Probe Dilution Buffer.
5′ and 3′ anti-EGFR Prox-Oligo probes were diluted in Assay Probe Dilution Buffer.
Indeed, according to Illumina's assay design tool, the majority of SNPs were unsuitable for Goldengate assay probe design.
BTK occupancy was assessed using an assay probe which bound the cysteine residue target in the absence of the drug.
The resulting 3′ anti-EGFR probe was diluted to 10 nM with Assay Probe Storage buffer and stored at −20 °C.
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RNA assay probes are listed in Supplementary Table 4. Lungs were isolated and pooled from three newborn mice.
For PCR, Taqman Gene Expression Assay probes were used (Supplementary Table 3), with reaction mixtures containing 1 μl cDNA, 10 μl TaqMan Universal PCR Master Mix Applied Biosystemss), 250 nM probes and 900 nM of primers from the Assays-on-Demand Gene Expression Assay Mix.
The final optimized PCR reaction conditions consisted of 20 ng of genomic DNA template, TaqMan Universal PCR Master Mix (1X), SNP genotyping assay probe-primer mix (20X), DNase-free water in a 5 μL reaction volume.
Assays for miR-495 quantification were conducted by using gene-specific TaqMan miRNA Assay Probes (Applied Biosystems, Foster City, CA).
TaqMan miRNA Assay Probes (Applied Biosystems, Foster City, CA) were used to quantify mature miR-181b according to the manufacturer's instructions.
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