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First-strand cDNA synthesis was carried out using the Superscript II RT kit from Invitrogen and its quantification was carried out in an Applied Biosystems 7500 Real Time PCR system using the SYBR Green I dye quantification assay (Power SYBR Green PCR master Mix).
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However, a half-sib family structure is not a requirement for a segregation analysis, and even for family sizes of one or two half-sibs with the sire being also assayed, power was increased to a higher value than when analyzing an individual alone.
For the in vitro assay, Cerezyme power was reconstituted with sterile water.
The NPV confirmed the assay's power in excluding TE.
In FRAP assay, reducing power was not detected in G. procumbens leaf callus (0.000 TEAC mg/g FW) whereby G. procumbens root exhibits the highest (1.103 TEAC mg/g FW) ferric reducing power.
For the same extract or standard with different concentrations, means in the same row with different letters (p t) were significantly different (p <0.05, ANOVA) A dissimilar pattern was observed when comparing the β-carotene – linoleic acid system with the pervious antioxidant assays (DPPH radical scavenging assay, reducing power assay and metal chelating assay).
The plant extract was tested for DPPH (1, 1-diphenyl, 2-picryl hydrazyl) radical scavenging, nitric oxide radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, antilipid peroxidation assay, reducing power and total phenol content.
The former Soviet dissident and political prisoner assays "the power of freedom to overcome tyranny and terror".
The Ferric Reducing Antioxidant Power assay (FRAP assay; refs).
The antioxidant activities of the decoction were investigated using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, nitric oxide scavenging assays and ferric ion reducing power assay.
The mean ± SD values of EC50 were 131.2 ± 36.1, 48.4 ± 12.1, 263.5 ± 28.3 and 87.70 ± 6.06 μg/ml for DPPH, hydroxyl radical, nitric oxide scavenging assays and ferric ion reducing power assay respectively.
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