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Table 1 Detection of results of CA72-4 in serum samples using QD-based ICTS Serum samples ICTS Validity (95 % CI) Positive Negative Total Roche assay positive (n = 70) 70 0 70 Sensitivity 100%% (91 100) Roche assay negative (n = 30) 0 30 30 Sensitivity 100%% (91 100).
Ovarian sections were imaged and TUNEL assay positive cells were counted manually using Image-Pro plusoftwarere.
Primers for assay positive control A. thaliana RNA and DNA RT-PCR reaction spike-ins, originally consisted of three primer pairs: 1) A. thaliana RCA gene primers for DNA targets, 2) A. thaliana Cab gene primers for RNA targets, and 3) A.thaliana rcbL gene primers for both DNA and RNA targets.
For each assay, positive and negative controls were used.
cEnzyme-linked immunosorbent assay (positive if PanBio units >11) (15 ).
In each RT-PCR assay positive and negative controls were included.
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Results: Of 124 women with spontaneous preterm births, only five (3.9%) had PCR assays positive forM genitalium.
Twelve or twenty-two pamplificationsicontainingontaining a combined total of 107 molecules of genomic DNA, were conducted simultaneously with analytical sensitivity assays, positive controls, and multiple negative controls.
Also, DNA primers encoded by MCTs have notably been used in multiplex PCR microbe detection assays; positive test results were verified by real time PCR [17], [18].
Goat polyclonal immunoglobulin G anti-α-actinin (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) was used as an intra-assay positive control Ab.
In all assays, positive and negative controls were performed.
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