Exact(5)
In this assay, performing cDNA synthesis in the absence of reverse transcriptase served as negative controls.
TaqMan allelic discrimination assay, performing in the ABI PRISM 7500 Sequence Detection Systems (Applied Biosystems, Foster City, CA), was used to detect genotypes of the chosen SNPs.
We investigated different angiogenic and profibrotic molecules in a tridimentional matrigel assay, performing co-cultures with endothelial cells (ECs) and bone marrow derived MSCs from patients and healthy controls (HC).
Even if the number of CIN2+ cases is relatively small in this study, we still see a contribution to the increasing sensitivity (75.0%–81.3) and specificity (94.6 96.6%) when including the same five HR HPV types in an assay performing an mRNA compared with a DNA-based assay.
These results suggest that the number of genome-specific SNP s) in the assay primers is positively correlated with the probability of a SNP assay performing as a codominant assay, and that a genome-specific SNP closer to the 3′ end of the assay primers is more useful for ensuring this behavior, but are not always effective.
Similar(55)
The cytotoxicity assay performed using MTT depicted ~75 80% cell viability.
The cytotoxicity assay performed using MTT assay as described by Mosmann [24].
Assay performed with GST alone constituted the negative control.
Assay performed with an empty vector constituted the negative control.
The EPC-CFU assay performed as described previously[4], [12], [28].
The EPC-CFU assay performed as described previously[17].
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