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The authors should generate mosaic clones and assay over time for clone size, expression of differentiation markers, and the frequency of stem cell loss.
The use of replicated samples for a high-throughput assay provides another measure of the precision of the assay over time.
We evaluated the results of the QuantiFERON-TB Gold In-tube (QFT-GIT) assay over time during the anti-TB treatment of adults with no underlying disease.
Data are presented to describe the stability of the assay over time as performed in a CLIA-certified clinical laboratory diagnostic setting.
Reproducibility of qHTS data has been demonstrated previously (Inglese et al. 2006), but given the particular importance of reliability in toxicity profiling, we performed additional studies of assay robustness and reproducibility both within an assay and between replicates of an assay over time.
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Temperature, species, cell lines, perfusion protocols, etc. are not standardized, and assays may change over time as new technologies become available.
The activities of these promoters in a xylose-utilizing yeast strain were measured by lacZ reporter gene assays over time during aerobic cultivation and microaerobic fermentation, both in xylose and glucose media.
Immune activation markers were assayed over time and changes reported as median and interquartile range.
To test whether the increase in viral set-point could reflect changing use of various quantitative HIV-1 RNA assays over time, we added type of assay to the model.
The association between testing false-recent and having a prior STARHS classification of RI may reflect the presence of "assay non-progressors" in this sample, or individuals who are repeatedly classified as RI by STARHS assays over time because of sustained low antibody levels[26].
For those paired data in which patients were assayed over time, a Wilcoxon matched pairs test was used.
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