Sentence examples for assay order from inspiring English sources

Assay order numbers for these genes were: PRL Hs00168730_m1 (this assay is recommended by ABI to give best coverage of all PRL splice variants); PRLR Hs00168739_m1; PLAU Hs01547050_m1; PLAUR-1 Hs00182181_m1 and ACTB Hs99999903_m1.

and 60°C for 1 min), with FAM fluorophore was used for the TaqMan gene expression assay (ordered online from https://products.appliedbiosystems.com).appliedbiosystems.com

Finally, we used a luciferase reporter assay in order to confirm the direct regulatory effect of miR-132 on levels of GAT1, PTEN and MeCP2 as positive control.

Our experiments are consistent with this timeframe, and cells required pretreatment with DMOG before the migration and invasion assay in order to induce WNT11 expression (Figs 1G–I and 4C,D).

Serial dilutions of standard PIP3 were subjected to the PI3K-HTRF assay in order to establish a regression line for calibration.

3T3-L1 celineine was assayed by Neutral Red Assay in order to determine in vitro toxicity of petroleum ether extract of BR-1 and BR-2.

Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to the assay, in order to allow the formation of enzyme-inhibitor complex.

The fully saturated medium was bubbled with air for 30 min prior to the start of each assay in order to ensure 100% oxygen saturation.

All samples from each experiment were analysed in the same assay in order to preclude inter-assay variability.

Here, we based our primary screen upon the LeTx assay, in order to generate a wide panel of PA-neutralizing monoclonal antibodies.

A YopE defective background was chosen for this assay in order to avoid a repressing effect on pore formation and thereby influence of ExoS translocation efficiency [74].