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In the lipid peroxidation assay, once again CFCTKPC performed the best, with an IC50 value of 0.5 ± 0.0 μM better than reduced glutathione (1.2 ± 0.1 μM).
All patients were routinely screened for BK viremia using a qualitative polymerase chain reaction assay once every 3 months, with additional tests ordered as needed based on clinical suspicion.
To compare with the MTT assay, once the plate was imaged with the CloneSelect Imager system, the MTT assay was performed; therefore, the results from the CloneSelect™ Imager and the MTT assay originate from the same plate and therefore are directly comparable.
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At 72 hrs., the time point that was typically assayed once injection protocols and knockdown strategies had been optimized, fra transcript levels were reduced by 80% on average (Fig. 3, p<0.0001), and a maximum of 90% knockdown was achieved in one replicate.
Worms were assayed once the progeny reached young adult age.
Individual samples were assayed once for all IVT analyses and twice for all DASL analyses.
These progeny were used for assays once they had reached adulthood.
The chlorinated humic acids were prepared freshly and chemically assayed once per week.
These progeny were used in the assays once they had reached adulthood.
Biomarkers specific to cardiovascular risk including high-sensitivity C-reactive protein (hsCRP) and pro-brain natriuretic peptide (Pro-BNP) were assayed once during the study period.
The culture medium was changed twice a week, and the cells (secondary astroglial cultures) were used for assays once they reached confluence (on Day 21 in vitro).
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