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This example of the integrity of RPM-Flu assay-generated target pathogen gene sequencing for detection and identification is reinforced by results for the other specific hemagglutinin, neuraminidase, and matrix gene sequences from the same RPM-Flu assay of the same aliquot of FluMist vaccine.
In every example the two M gene sequences of an individual specimen, generated from the M H1N1) and M H3N2) gene detector tiles, are consistent with the A/HN subtype directly indicated the HA and NA gene sequences generated in the same RPM-Flu assay of the same specimen.
(d ) Viability assay of the same cells as in (c).
The G2 MN assay of the same cell probes shown in Figure 4 revealed that previous RT exerted strong effects on both baseline and in vitro radiation induced MN yields.
We also carried out a methylated DNA immunoprecipitation (MeDIP) assay of the same FRG1 promoter regions (FRG1 A and FRG1 B) to analyze the DNA methylation status of the promoter, and mapped a region of DNA methylation in correspondence with the YY1 binding site (compare FRG1 A and FRG1 B in Figure 1f part ii).
When an assay of the same type was run on the same resin, but the stress condition was varied (e.g., microwave stress versus QUV stress in Figures 2 and 3), then the two means and SD's of these two sets of repeated assays were sometimes compared using a two-tailed Student's T-test.
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Where more than one type of assay for the same index test was used (for example, Abbott or Biosite BNP), the more conservative estimate of sensitivity was extracted and included in the review.
This genotypic mismatch rate falls within the 0.10% to 0.30% mismatch rate reported by Affymetrix for replicate genotyping assays of the same purified DNA sample (after equivalent quality control).
Their objective was to produce specific monoclonal antibodies (MCAs) against aromatase that are capable of detecting aromatase through immunohistochemistry of 10% formalin-fixed paraffin embedded sections of breast carcinomas and establishment of scoring systems which would be best correlated with biochemical assays of the same specimens.
Interestingly, 92% of the edges in the network connect assays of the same experimental type.
The first of these concern what we term technical replicates (independent assays of the same reagents).
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