Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis.
The assay of the effects of low extracellular divalent cations on the cell viability of 293-TRM7 expressing cells was performed as described with the following modifications [36].
Thus, five compounds from these three clusters were selected [based on their commercial availability, cost and purity (≥95%)] for experimental assay of their effects on hIKK-2 activity.
Using knock-out mice we chose to study cataractogenesis in the lens and oncogenic transformation in mouse embryo fibroblasts to assay for effects of ATM deficiency.
A stable transfected human kidney epithelial cell line HEK293W was created as described and used for assay of inhibitory effect of compounds on Wnt signaling [14].
TSP1 inhibits NO/cGMP-stimulated VSMC adhesion on collagen [40], and we used this adhesion as a functional assay of the effect of Aβ on cGMP production.
The MacGreen (CSF1R-EGFP) transgenic reporter chick embryos [ 21] provided a convenient assay of the effect of CSF1.
Based on the results of MIC determination experiments, the sublethal concentrations of both bioactive compounds were identified and used during the assay of conjugative effect.
For the assay of conjugative effect, sublethal concentrations of SL and cefaclor were decided to be 500 μg/mL and 50 μg/mL, respectively.
Further, such a study could also incorporate an assessment of attentional function both before and after training, providing a more direct assay of the effect of the intervention on attention than the between-subject approach used in the current study.
