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b ALP assay of cells over different days.
Each point indicates the mean ± SD of values obtained from the assay of cells from six replicate microtiter plate wells.
Open image in new window Fig. 7 MTT assay of cells on A and B samples after 3 days of incubation.
When PRG scaffold was increased from 0 to 40%, there was a ∼3-fold increase of cell proliferation as determined by the DNA concentration assay of cells from PRG scaffold.
Top: SpR assay of cells.
The MTT assay of cells viability after treatment with increasing doses of anticancer drugs showed significant variations in IC50 doses between the examined cell lines.
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(D) CCK8 assay of cell proliferation with Hcy stimulation with or without ER stress inhibitor PBA or (F) ER stress stimulator DTT.
Scanning electron microscope (SEM) observation and fluorescent DiO labeling were carried out to reveal the attachment and growth of hASCs on the two ceramic surfaces, while the quantitative assay of cell proliferation with time was detected by DNA assay.
For routine assay of cell-cycle progression, cells were seeded in 96-well microtitre plates at 4×103 cells/well in a final volume of 100 µl medium.
Although we showed a biological role for a number of NATs in the assay of cell viability and proliferation, this assay likely covers only a small region of "phenotype space".
We also used an assay of cell viability.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com