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The assay needs to be evaluated in a clinical context and integrated in standard diagnosis of flaviviral diseases.
In addition, for confirmatory studies to distinguish primary apoptosis-inducing agents from primary necrosis-inducing agent, the assay needs to be performed in a real-time mode.
Knowing that the water addition can take place in a spontaneous manner, the significance of the spectrophotometric assay needs to be doubted.
To ensure its presents, additional investigations on the possible hydratase in A. denitrificans are necessary including variation of the growth conditions and a screening methodology different from the coupled spectrophotometric assay needs to be employed to minimize the risk of producing false positive results.
However, to have clinical value, such an assay needs to be accurate, reliable and relatively convenient.
However, this assay needs to be fully validated given the multiple splice variants.
Similar(41)
3. Information on the source of the SM1/23 mAb and a reference for the cell death assay need to be included in the Methods section.
Molecular assays are rapid and sensitive but due to their low level of multiplexing usually a number of different molecular assays needs to be performed sequentially to achieve definitive diagnosis.
To determine the specific activity of CLEAs the mass of PP present in the aliquot of CLEAs assayed needed to be inferred (Column "Mass of PP in aliquot of CLEA assayed" in Table 3).
Because a large number of different immune-assays needed to be performed on culture supernatants derived from the limited pediatric blood volumes drawn, some samples were consumed before all analyses could be completed.
If new assays need to be performed, they can be carried out using standardised laboratory methods.
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CEO of Professional Science Editing for Scientists @ prosciediting.com