The phrase "assay modification" is correct and usable in written English.
It can be used in contexts related to scientific research, particularly in chemistry or biology, where modifications to an assay (a test or analysis) are being discussed.
Example: "The researchers implemented an assay modification to improve the sensitivity of the detection method."
Alternatives: "test alteration" or "analysis adjustment".
In addition to outlining established methods, an assay modification is proposed to measure fast autodephosphorylation rates with radioactivity, and optimization of the fluorescence/pH jump method is described.
Using this assay modification, higher concentrations of compounds that interfere with C. elegans development and growth were tested in the starvation assay (Figure 1C and Figure 4B).
Factor VIII and FIX inhibitor assessments (Nijmegen assay modification of the Bethesda assay 14), complete blood count and coagulation tests were performed every 3 months; clinically significant abnormal values were reported as AEs.
In order to avoid the overlap of green and red fluorescence in a dual heterozygous assay, modifications were made to the previously described assay.
Assay modifications were as follows: Platinum Taq DNA Polymerase (Invitrogen, Brazil) was used as the master mix, and for the rrs assay the Mg concentration was reduced from 4.50 to 4.25 mM, the forward primer reduced from 1 µM to 0.25 µM, the reverse primer reduced from 1 µM to 0.50 µM and the probe reduced from 0.20 µM to 0.05 µM.
HA interference can be minimized by assay modifications or sample pre-treatment.
Some assay modifications were necessary for accurate quantitation of viral load.
The impact of assay modifications on the accuracy of clinical decisions still has to be determined.
Therapeutic compounds, however, can directly interfere with assay design and assay modifications are required dependent on the therapeutic approach.
See ELECTRONIC SUPPLEMENTARY MATERIALS for details. Assay modifications were made throughout the method development process; final assay conditions are described in " Method Validation" below.
The results of most of these experiments are recapitulated in " Method Validation", but two important assay modifications are described in detail below.
