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Thus, it is necessary to develop diverse assay methods for detecting sulfatase activity.
Accordingly, the development of assay methods for those two drugs is desirable for the purpose of quality control.
Furthermore, the use of enzymatic assay methods for diagnosis has been facilitated by the developments in genetic engineering which made it possible to overproduce enzymes inexpensively.
Other assay methods for olopatadine and its metabolites in human plasma by HPLC with electrospray ionisation tandem mass spectrometry were reported (Fujimaki et al. 2006; Fujita et al. 1999).
The classical assay methods for PepP activity determination present some problems ([Yoshimoto et al. 1988b]; [McDonnell et al. 1997]; [Lasch et al. 1988]; [Fleminger et al. 1982]; [Holtzman et al. 1987]; [Hawthorne et al. 1997]; [Stöckel-Maschek et al. 2003]).
This strategy allows precise and efficient analyses of enzyme kinetics not possible with the conventional assay methods for the glycosyltransferases that usually require separation of products from the reaction mixture.
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The luminescent reaction of the firefly has been used as an assay method for the determination of adenosine triphosphate (ATP), an important metabolic substance used by all living cells in numerous reactions in which energy is either stored or expended.
This novel assay method for the quantification of lysozyme is highly specific, sensitive, accurate and reproducible.
The current work deals with the screening of conditions controlling spectrophotometric SIA and developing a new assay method for diclofenac.
The MTT is a reliable assay method for measuring cell viability in different substrates, especially in rigid and porous scaffolds.
Toxicolor (Seikagaku Corporation, Tokyo, Japan), which is a chromogenic Limulus amebocyte lysate test, was used as an assay method for LPS.
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