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The results from these simulations were then used to estimate the Pu assay capabilities of the FNMS in terms of counting time, assay mass, and assay mass variance, using assay mass variance as a figure of merit.
Most notably, the time required to perform an assay to an equivalent assay mass variance is greatly reduced with a fast-neutron system, by more than an order of magnitude compared with that of the thermal-neutron system, due to the reduced probability of random summing with the fast system.
The maturity of high-throughput experimental techniques and computational prediction, such as two-hybrid assay, mass spectrometry experiments, and the protein chip technology, make it possible to construct large-scale protein-protein interaction networks (PPINs) of many species.
We, therefore, systematically checked clustered distorted SNP markers from the 12 k assay in a larger sample (380 F2s) with a targeted medium-throughput genotyping assay (Mass Array, Sequenom).
In vitro sumoylation assay, mass spectrometry detection of sumoylated lysines, and electrophoretic mobility shift assays (EMSAs) were performed as described previously, except that the EMSA used a 5% polyacrylamide gel in 0.5 × Tris-borate-EDTA and 6 nM DNA substrate (Sarangi et al., 2014).
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Here we report using GST pull-down assay, mass-spectrometry and co-immunoprecipitation that a heterotrimeric complex of DNA-Dependent Protein Kinase (DNA-PK), consisting of Ku70, Ku80 and DNA-PK catalytic subunit (DNA-PKcs), is a novel interaction partner for AIRE.
Because cell extrusion typically promotes epithelial cell death (Eisenhoffer et al., 2012; Marinari et al., 2012) and because S1P2 depletion did not affect the proliferation rate in a yellow tetrazolium MTT (3- 4, 5-dimethylthiazolyl-2 -2, 5-dimethylthiazolyl-2 -2omide) 5-dimethylthiazolyl-2 -2y 5-dimethylthiazolyl-2 -2apoptosis.
In vitro methods include binding assays, Mass Spectrometry (MS) based methods and cellular assays such as intra-cellular cytokine staining, proliferation assays, and Elispot assays.
In past decades, significant progress has been made to develop alternative in vitro detection methods such as immunoassays, endopeptidase activity assays, mass spectrometry, cell-based assays, and so on [24].
Using a micro BCA assay, the mass of BSA released was determined.
For pharmacokinetic analysis, plasma sampling was performed during the first course and assayed using a validated high-performance liquid chromatographic assay with mass spectrometric detection.
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