Mutations interfering with WAVE-Lpd binding impaired cell migration in wound closure assays (Law et al., 2013), demonstrating the functional relevance of this interaction.
NP has been shown to have equal or even more estrogenic activity than BPA in in vitro and in vivo assays (Laws et al. 2000).
The authors thank Jean Chang for processing the RNA samples and performed microarray and PCR assays, and Lynn Law and Marcus J. Korth for valuable feedback on the manuscript.
The purity of the PHA was measured by the crotonate assay ([Law and Slepecky 1961]) and gas chromatography (GC) analysis (Divyashree et al. [2009]).
The present assay enables measurement of growth laws in a batch culture, including the stages where substrate is rapidly depleted by cells nearing stationary phase.
Protein concentration was measured by BCA assay and by absorbance at 280 nm, using Beer's law.
In short, samples were analyzed by using a rapid screening assay (PCR) to provide preliminary information to health-care providers and law enforcement.
F oF 1-ATP-ase (Complex-V) activity was assayed by measuring the release of Pi from ATP by the method of Law et al.[ 17].
Check for state laws and local laws.
Mechanistically, compound:target binding occurs at a single site and follows the law of mass action that is reflected by the sigmoidal dose response pattern seen in many assays (Balakrishnan, 1991).
