Sentence examples for assay is started from inspiring English sources

The assay is started by mixing DUB and diUb solutions, and 5 μl aliquots are taken at indicated time points.

This could look like an apparent inhibition of the complex I-dependent reaction if the assay is started with a partially de-activated enzyme.

Plates were sealed in plastic bags and stored at 4°C until the assay was started.

The XTT assay was started 24 h after the medium change when new medium supplemented with nanoparticles was added.

The assay was started by the addition of supernatant of 293FT cells and the lysozyme absorbance was recorded every 30 s over 20 min using a spectrophotometer.

Cultures were then incubated for 5 d in vitro (DIV) at 37°C and 7% CO2 before the neurotoxicity assay was started.

The assay was started by adding 60 µM decylubiqunol and absorbance recorded for one minute, taking the initial 30 seconds rate for calculation.

The nitrite assay was started by adding the sample (360 µg proteins in 50 µl) to 50 µl sulfanilamide solutions in 96-well plates in duplicate.

The assay was started by the addition of mitochondria and the change in absorbance at 340 nm (ε = 6.22 mM−1cm−1) due to NADH oxidation in the presence of ubiquinone-1 was recorded for 1minute, after which 5 µg/ml rotenone was added and the absorbance recorded for a further 2 minutes to allow for the quantification of the rotenone-sensitive activity.

The assay was started within 48 h.

Aliquots were incubated for 5 min at 30 °C before uptake assay was started.