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Further evaluation of the assay is needed in large-scale trials which can also allow for some modifications to streamline the procedures for routine use.
To enable better diagnosis and inhibit the further spread of this re-emerging virus a quantitative (q) real-time reverse transcription polymerase chain reaction (qRT-PCR) assay is needed for early detection of CiLV-C when the virus is present in low titer in citrus leprosis samples.
In order for medicinal chemists to tune solubility of lead compounds, a rapid assay is needed to provide solubility data that is accurate and predictive so that it can be reliably used for designing the next generation of compounds with improved properties.
This means a strong stimulus and/or a highly sensitive assay is needed to reliably detect a response.
Spectrophotometric assay is needed for detecting carbapenemase activity.
Clearly, for assessing exposure to B. pseudomallei, an accurate, inexpensive, simple serologic assay is needed.
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HOTAIR also regulates BCSCs, and microarray analysis reveals HOTAIR overexpression upregualtes the genes related to stemness and EMT, such as CD44, STAT3, ALDH2, ZEB1 and VIM, but the tumor initiating frequency in vivo assay are needed to demonstrate the role of HOTAIR in regulating BCSCs further (Padua Alves et al., 2013).
These false negative findings suggest that further modifications of the ALS assay are needed to improve sensitivity.
An assay was needed to quantify the nanovesicle formation.
First, a new assay was needed to follow this methyl transfer reaction.
Rigorous field evaluations of this assay are needed and we discuss the associated challenges in study design.
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