The phrase "assay integrity" is correct and usable in written English.
It can be used in contexts related to testing or evaluating the reliability and accuracy of a process, system, or material.
Example: "The laboratory must assay integrity to ensure that the results of the experiments are valid and trustworthy."
Alternatives: "evaluate integrity" or "test integrity".
Growth curve assay, membrane integrity assay, scanning electron microscopy (SEM) and confocal scanning laser microscopy (CSLM) revealed different mechanisms of biofilm inhibition in E. cloacae and S. mutans.
Several observables were quantified: mitochondrial activity through WST-8 assay, membrane integrity with LDH release measurement, apoptosis by flow cytometry and DNA fragmentation with Tunel test.
The assay measures integrity of DNA using long PCR targets, in this case 8.9 kb in length, which is about 2/3 of the entire mtDNA.
None of these treatments had a marked effect on viability of cell cultures by the end of the exposure as measured by the trypan blue exclusion assay; nuclei integrity was maintained in I-IUdR-treated cells providing no evidence for apoptosis as observed by DAPI nuclei staining (data not shown).
To ensure quality of mitochondrial preparations, samples were subjected to various assays for integrity and functionnality including cytochrome oxydase accessibility, respirometry and FSC/SSC FACScan (BD Bioscience, Germany) analysis in the presence or absence of Mitotracker™ green (ΔΨm insensitive) and Mitotracker™ red (ΔΨm sensitive) as described in [30].
Isolated mitochondria were subjected to various assays for integrity and functionality as described.
To ensure quality of mitochondrial preparations, samples were subjected to various assays for integrity and functionality including forward scattter/side scatter FACScan (BD Bioscience, Mannheim, Germany) analysis in the presence or absence of Mitotracker™ green (DYm insensitive, Invitrogen, Darmstadt, Germany) and Mitotracker™ red (DYm sensitive).
At various time points after PDT treatment, the plates were separately assayed for necrosis (cytotoxicity) using the fluorescence-based Cytotox-ONE Homogeneous Membrane Integrity Assay kit (Promega) and apoptosis using the Apo-ONE Homogeneous Caspase-3/7 Assay kit (Promega, Madison, WI, USA).
Interestingly, membrane integrity assay showed that PtNPs treatment caused perturbation of inner membrane with leakage of nucleic acid similar to 48% leakage seen with Triton-X-100 treatment and leakage of protein comparable to 29% of that observed in Triton- X- 100 treatment (Table 5).
