Sentence examples for assay input from inspiring English sources

PBMCs were titrated to cervical appropriate cell numbers to determine minimum required assay input cell numbers; qPCR retained sensitivity with input of at least 2.5×104 PBMCs.

Two independent cultures of cells were used for the FAIRE assay (input and FAIRE-treated cells).

The measured COL2A1 concentration is therefore not sensitive to any potential methylation of CpG dinucleotide motifs and can therefore be used to measure the assay input of DNA.

At 48 hours following transfection, precleared chromatin was stored as assay input or was incubated overnight at 4°C with 5 μg of rabbit anti-SOX9 antibody (Abcam) or normal rabbit IgG (Cell Signaling Technologies).

For the analysis of BrdU-labeled DNA obtained from ChIP assays, input and ChIP DNA were eluted in 50 μl water.

In turn, the SRI researchers are providing the U.S. EPA and other federal partners additional review of selected assays and input on new assays needed to cover biological space not yet represented in the current ToxCast™ and Tox21 assays.

The reason for this is based on the fact that a pyrosequencing assay requires input of a DNA sequence to read the methylation levels while synthesizing the DNA strand according to the user-entered sequence.

For plasma DNA assays, the input level of DNA, corresponding to 555 µL plasma per PCR assay, is variable.

DNA samples, from ChIP assays or input DNA (0.1% of the sample used for the ChIP assay), and primers (200 nM, IDT) were added to the additional rtPCR reagents (EXPRESS SYBR® GreenER™ qPCR kit) in a 20 μL reaction volume and subjected to the following cycle: 50°C/2 min, 95°C/2 min, 40× (95°C/15 sec, 60°C/1 min), using ABI 7900 HT or Cepheid Smart rtPCR instruments.

DNA samples, from ChIP assays or input DNA (0.1% of the sample used for the ChIP assay), primers (1 μM, IDT and DMSOO (5%) were added to the additional PCR reagents (AmpliTaq® Gold Polymerase PCR kit and dNTP mix) in a 50 μL reaction volume and subjected to the following cycle: 95°C/9 min, 43× 95°C/11 min, 60°C/1 min), and 60°C/10 min, using 96-well plates and an Eppendorf Mastercycler®.

In this assay, three-input concatenated logic gates operating as a biocomputing keypad-lock security system also have been successfully constructed.