The maximum difference between assays in two different laboratories is expected to be 4.1%.
Each genomic DNA sample was assayed in two different gDNA amounts (10 and 50 ng), running each amount in duplicate and the entire assay was repeated twice.
All measurements of ligand concentration (ng ml−1) were computed averages from duplicate assays in two different enzyme-linked immunosorbent assay plates.
It should be highlighted that the samples in Study 1 and Study 2 were analyzed using different assays in two different laboratories.
Human genomic DNA was digested with the restriction endonuclease Alu I and assayed in two different tubes, each containing one of the two allele specific OCP.
We also explored the effect of both inhibitors on cell viability using live/dead and cell proliferation assays in two different cell lines.
For all PBoL insertions, lacZ expression in ovaries was assayed in two different genetic backgrounds (Canton y and w ) to take into account possible background effects on transgene expression.
Although we cannot directly compare selectivity of compounds against GlpG and chymotrypsin, because they are assayed in two different formats, we can deduce high selectivity for GlpG over chymotrypsin for compounds that already show selectivity for AarA in the fluorescent assay.
Anchorage-independent growth was evaluated by the soft agar colony count assay in three different experiments.
