The phrase "assay in the same" is not correct and does not convey a clear meaning in written English.
It seems to be an incomplete or incorrect expression, and without additional context, it is difficult to determine its intended use.
Example: "The results of the experiment were inconclusive, so we need to assay in the same conditions again."
Alternatives: "test under the same conditions" or "evaluate in the same manner."
These findings were further confirmed with conventional luciferase assay in the same samples.
Further, this assay is compatible with high throughput drug screening since all that is required for measurement is coelenterazine, its substrate, and a plate luminometer which measures its reaction in few seconds and there is no need to remove the conditioned medium (since >95% of Gluc is secreted) if one prefers to assay in the same well where cells are plated and not overtime.
To verify the results, we performed caspase activity and DNA laddering assay in the same condition.
An interesting question not directly addressed in this article is the reproducibility of the assay in the same individual over time.
Therefore, we used flow cytometry analysis to simultaneously quantify apoptosis and the SA- βGal activity assay in the same cell population.
Indeed, the biomarker panel of 16 citrullinated autoantigens identified RA patients with a sensitivity greater than that achieved by the commercial CCP assay in the same cohort.
Meanwhile, an equivalent number of cells coexpressing BNMnAS, BNMcAS, and SspB in the dark, and cells coexpressing mN159, mC160, and SspB under 470-nm light were used as controls and assayed in the same manner.
The dataset employed is homogeneous, as all peptides were assayed in the same conditions and thus is of great value to test the parameters used in this study.
We then obtained a second set of 17 additional samples, which also showed quantifiable migration rate differences when assayed in the same way.
Therefore, it was cloned into our expression vector, both as a truncated OmpA consisting only of the TM domain and as a full-length protein, and assayed in the same way as the FLAG/myc insertions.
