Sentence examples for assay in order from inspiring English sources

Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to the assay, in order to allow the formation of enzyme-inhibitor complex.

3T3-L1 celineine was assayed by Neutral Red Assay in order to determine in vitro toxicity of petroleum ether extract of BR-1 and BR-2.

The fully saturated medium was bubbled with air for 30 min prior to the start of each assay in order to ensure 100% oxygen saturation.

Serial dilutions of standard PIP3 were subjected to the PI3K-HTRF assay in order to establish a regression line for calibration.

Moreover, the tissue of tumors should be observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in order to compare the amount of apoptotic cells (Figure 2b).

Cellular metabolic activity was assessed by means of the MTT assay in order to evaluate the activity of mitochondrial succinic dehydrogenase by measuring the amount of formazan produced by this enzyme [27].

Belyanskaya reported that addition of one further centrifugation step at the end of the MTT assay in order to lyse the cells and discard the cellular components and CNT particles could reduce interferences caused by the CNTs.

A response surface methodology was applied to design and optimize the assay in order to maximize the differences on the expected effect and to minimize the number of experiments.

The aim of the present work was to develop a simple and inexpensive PCR-SSCP assay in order to discriminate the CG/AA alleles in exon 8 of the DGAT1 gene.

The baseline level of proHGF and HGF derived from the medium and/or endogenous proHGF and HGF were firstly detected by an ELISA assay, in order to establish that they would not affect the measurement of mESCs pluripotency and differentiation in our assay (Fig. S3).

All samples from each experiment were analysed in the same assay in order to preclude inter-assay variability.