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The effects of Dox and AD198 on cell apoptosis were determined by caspase 3/7 assay, generation of reactive oxygen species (ROS), and Western Blotting (WB) analysis.
The present study was consistent in terms of an increase in ovariole number in selected females, irrespective of the assay generation or of the number of generations removed from the selection regime.
Similar(58)
hs-cTnT assays have been shown to provide a better early diagnostic sensitivity for AMI in the ED compared with the previous cTn assay generations [ 2, 100, 101].
Future assay generations may address this lack of sensitivity by designing a micro-fluidic cartridge where splitting the specimen into different volumes so that the volume for IL-1β assay will be set optimally for the purpose of improving sensitivity.
Details about spectra acquisition, MS settings, SRM-assays generation and optimization can be found in Additional file 12: Supplementary Methods.
RR (in vivo, in vitro and cell culture assays, generation of rx2 mutants) and LC (lineage-tracing analysis, transplantation assays) performed the experiments with contributions from TT (transplantation assays), DI (Rx2 antibody and GFP fusion proteins), J-PC (design of rx2 talens) BW (rx2 mutants), and JRM-M (rx2 Tub-GFPP trx2 Tub-GFPne).
Various Rac1 activation assays and functional assays, such as migration assays, in vitro wound healing assays, cell proliferation assays, and ROS generation assays, were also performed.
This also highlights another theme of our review, the necessity to develop a better means of measuring NO, both to assay NO generation and the site of its generation.
Once the organism is identified based on such an assay, the generation of new MALDI-TOF MS reference spectra will allow identifying this species or isolate in the future (Fig. 1b).
Dilutions of ricin alone were included in each assay for generation of a standard.
Finally, the quantitation of the viral RNA was performed in a TaqMan assay after generation of cDNA.
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