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However, determination of analytical assay framework is important for ready-to-use real-time PCR reagent systems for rapid, quantitative and accurate detection of bioterror pathogens such as Bacillus anthracis.
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Possibly, the MetaboLights database that is part of the ISA (Investigation, Study, Assay) commons framework can fill this gap.
The emerging standard for biological experimental metadata is the investigation, study, and assay (ISA) framework (http://www.isacommons.org).org
Structured information is provided according to the Minimal Information About T-cell Assays Reporting Framework for human T-cell assays [ 14, 15].
The authors acknowledge the concept of the Minimal Information About T cell Assays (MIATA) framework, which was recently published [ 21, 22].
We acknowledge the concept of the minimal information about T cell assays (MIATA) reporting framework for human T cell assays [ 18].
We considered two widely used sets of phase characteristics: morphometry, which is traditionally used in the field, and behaviour, which is widely used since the recent establishment of an assay and descriptive framework for locust behaviour [reviewed in [ 26]].
Taken together, the comparison of 16 different HIV-1 neutralization assays within the framework of an international network, NeutNet, comprised of 15 laboratories, led us to the conclusion that at present no single assay can be recommended for use alone as a potential correlate of vaccine efficacy.
For that purpose, we have developed a new data format termed Standardized Description of Operating Procedures (SDOPs), which provides an assay-specific descriptive framework and enables direct and detailed comparison of procedural parameters.
We present clinical validation of the assay and a detailed framework for design and validation of similar clinical assays.
The liquid culture format of the assay provides a scalable framework for the testing of chemical, genetic, and environmental perturbations in C. elegans.
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