We need an accurate assay rather than formulas to measure free and bioavailable concentrations.
PCR amplification efficiencies were calculated for each candidate reference gene assay using the formula E = (10-1/slope - 1) × 100, using the slope of the plot of quantification cycle (Cq) versus log input of cDNA (10-fold dilution series).
Percent inhibition was calculated by following formula; This assay was performed as illustrated by Dorman et al. [ 15].
MTT assay demonstrated that the formula up to 60 μg/ml and GA up to 10 μg/ml did not affect cell viability.
For dose response curve fitting in the DPPH assay and linear function formula of dose response in phosphomolybdenum assay analysis of variance (ANOVA), Tukey's post-choc test were performed (GraphPad Prism 5 Pad Software Inc, La Jolla, USA).
The percentage fluorescence in the test samples in reaction with control samples was calculated by using following formula: For assay, 5 nM supercoiled pBluescript d-s plasmid DNA and 10 U of topoisomerase-I active enzyme were used in each reaction well.
Activity of the LDH release is expressed as percentage with respect to the total LDH content (LDH in the supernatant + LDH inside the cells) according to the following formula: To assay ROS formation, 2,7-dichlorodihydrofluorescein diacetate (H2DCF-DA), a non-fluorescent lipophilic reagent, was used.
The TE frequency was measured using a T7 endonuclease I assay and analyzed using a formula described in 'Materials and Methods'.
Because of potential assay fluctuations over time, these formulas may not always capture the appropriate correction factor at the specifictimeof use; nonetheless, they provide a general idea of the relationship between assays.
The DNA methylation levels analyzed by the COBRA assay were calculated using the formula (1) Estimated methylation degree % = 100 × I C I C + I U C, where I C and I UC represent the sum of the intensities of digested and undigested bands, respectively.
Combination of the enrichment, with the PCR assay can detect 1.5 CFU/100 g infant formula of K. pneumoniae within 48 h.
