Time-dependent concentration profiles were obtained from tissue samples incubated in the Transwell assay, for times ranging 30 minutes - 6 hours.
In order to accomplish this, we adopted an assay for timed exposure of animal cap to anterior endoderm, in which we removed or peeled animal cap from anterior endoderm after defined time.
Characterisation of microbial pathogens beyond the species and subspecies level, that is, subtyping, requires more than one marker and these markers are usually combined in a multiplex assay for time-effectiveness of the assay.
Each assay, for each time point, was repeated three times.
Statistically significant differences were only encountered in the ELISPOT assay for this time point when the 16 kDa and Ag85B stimulations were used (one-way ANOVA, p<0.05).
Polymerase chain reaction was performed with a TaqMan Assay for real-time PCR (7500 real-time PCR system; Applied Biosystems) following the manufacturer's instructions.
On-chip human microvasculature assay for real-time visualization and quantitation of tumor cell extravasation dynamics.
Herein, we describe a facile and sensitive fluorescent assay for real-time studying endonuclease activity and inhibition based on the photo-induced electron transfer (PIET) between deoxyguanosines and a fluorophore.
Thus, we developed a simple and quantitative assay for real-time monitoring of HCV core protein inhibitors in mice.
The PamChip® arrays consist of 48 peptides encompassing the LxxLL motifs of 19 different coregulator proteins ([63], Koppen et al. 2007. Micro Array assay for Real-time analysis of Coregulator-Nuclear receptor Interaction. Manuscript submitted).
To exemplify that chromobodies can complement and extend existing diagnostic approaches, we set up a first cellular assay for real-time monitoring of apoptosis in HeLa cells.
