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Meanwhile, the cell migration ability was assessed by transwell matrix penetration assay for the same treatment.
Compared with previously published real-time Taqman RT-PCR assay for the same gene target of PPRV [ 7, 9, 10] the present assay was more sensitive.
This amount of intact EVs corresponded to the fraction of 7D11-insensitive EVs determined by plaque assay for the same supernatants (data not shown).
Hoechst/PI assay for the same SSP dose showed a graduated increase in apoptosis up to 24 h, at which point maximum apoptosis was observed.
SCCVII tumours in C3H mice were analysed for pimonidazole binding using flow cytometry and an iterative curve-fitting procedure, and the results were compared to the comet assay for the same cell suspensions.
Where more than one type of assay for the same index test was used (for example, Abbott or Biosite BNP), the more conservative estimate of sensitivity was extracted and included in the review.
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Interestingly, SLK kinase assays for the same time course showed that its activity was low in cells with large adhesions or high levels of pY397-FAK (figure 4D).
Comparing results of the two qPCR assays for the same sample, we saw that the values of nNEO had a minimal variability.
By performing some assay optimization, assay replacement, or by including multiple assays for the same genomic target, our capture success rate can be increased, which is important for diagnostic applications.
Differences have been observed in the ability of assays to correctly detect or exclude recent infection [ 15, 16] and in the results reported by different assays for the same samples.
The reproducibility of the clustering analysis was confirmed by repeating the paired dye-swap hybridizations for one of the sixteen strains, randomly selected, and by verifying that independent assays for the same strain grouped together in the dendrogram (not shown).
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