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While on the face of it this seems to be self-evident, the composition of diets that are used vary greatly and no effort is currently made to control or even assay for such substrates.
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Well designed statistical approaches that quickly identify optimal conditions for a given assay could assist efficient completion of the laboratory assays for such studies.
However, this hypothesis remains open and requires further investigations, as we did not evaluated specific functional assays for such processes.
Imaging plays an important role in assays for such disease models although, to date, most of the readouts have been concerned with morphology and ultimate phenotypes, which can be difficult to directly correlate with cellular responses.
The more conserved sequence will also lead to primers or probes hybridizing to both the gene sequence that contains the SNP as well as any conserved paralogous sequence, thereby decreasing the success rate of assays for such SNP [ 22].
This evaluation of tripeptide frequencies at the level of the protein family instead of the individual protein then allows for the specific assaying for such signatures that occur as genome-wide over-represented signals due to generalized structural or functional requirements in C-terminal biology.
This is expected to speed up drug development timelines by early evaluation of target effects and the usefulness of the assay systems for such targets.
Only few methods have been reported in the literature for the assay of such mixture, using chemometric assisted spectrophotometric methods [3, 4] and HPLC methods [3 5].
To the best of our knowledge, this is the first study for the assay of such additives in dairy products available in Bangladesh.
While the former study used a Biosource assay, the latter used the gold standard LCMS assay to measure vitamin D. Aside from assay differences, reasons for such wide discrepancies remain unexplained.
We specifically performed survival assays for two such proteins: a sodh-1 mutant and the lec-1 mutant.
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