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Following discordant analysis, the sensitivity of the ProPneumo-1 assay for pathogens, C. pneumoniae or M. pneumoniae, was 100%.
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Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring.
This work aims to design a unique RT-qPCR assay for pathogen detection in the three salmonid species reared in Chile.
While traditional assays for pathogen detection and typing represent the gold standard, they alone, cannot meet the future needs for rapid, sensitive, specific and simple methods.
The network collects samples in different locations, which are tested using a lateral flow immunological assay for different pathogens, only when the weather conditions are suitable for infection.
The ideal test should also be part of a multiplexed assay for other pathogens causing acute undifferentiated fever, such as malaria [ 17].
The specificity of the GeXP assay for each pathogen was examined using single cDNA/DNA template.
As a model assay for MIP pathogen diagnostics, we chose to target the Human Papillomavirus (HPV), which is well known for its cancer-associated cervical infections and for the existence of multiple genotypes [20].
To evaluate the detection limit of the CPDC assay for each pathogen, 2 ng to 20 fg of DNA per reaction was prepared.
In conclusion, these results demonstrate the utility of the RAPD plus PCR approach in the development of genetic diagnostic assays for fish pathogens.
Here, we detail how collections of bacterial genomes from a particular species (population genomics libraries) have already been used to improve the design of several diagnostic assays for bacterial pathogens.
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