Sentence examples for assay for contamination from inspiring English sources

A negative control (no template) and a positive control (DNA template from M. tuberculosis H37Rv) were included to assay for contamination of the reagents and successful PCR amplification, respectively.

The standard curve was generated by performing serial dilutions of the armored HIV RNA and assaying each dilution twice per RT enzyme, while using a non-template control in each assay to check for contamination.

This will result in the discovery of fixed differences in the nuclear genome between Neandertals and current humans that can serve as future direct assays for contamination.

Each run included a control free of template to test the assay reagents for contamination.

Mock reactions with no RT were done on each sample to assay for DNA contamination.

Prepared libraries have undergone a quality control using an Agilent 2100 Bioanalyzer: DNA 1000 Labchip, Quant-iT dSDNA HS Assay for quantification and Quant-iT RNA Assay and Quant-iT Protein Assay for percentage contamination check, using an Invitrogen Qubit® Fluorometer.

As an additional assay for possible contamination, we amplified the SSU rRNA gene region from DNA extracts by PCR with eukaryotic-universal SSU primers [SR1 (5'-TACCTGGTTGATCCTGCCAG-3') and SR12 (5'-CCTTCCGCAGGTTCACCTAC-3')], then sequenced amplicons directly.

No-template PCR controls (consisting of master mix, primers, and water) and sham digest controls (template consisting of water subjected to DNA extraction) were run with each PCR assay to monitor for contamination.

All SP-A preparations were assayed for endotoxin contamination using the Limulus amebocyte lysate assay (BioWhittaker, Rockland, ME) per the manufacturer's instructions.

RNA samples were assayed for DNA contamination by PCR without prior reverse transcription.

There are PCR methods available that target other regions of the cauliflower mosaic virus that can serve as control assays for virus contamination [ 16, 17].