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Using the Roche Linear Array assay for comparison, we tested twenty-eight samples (16 primary OPSCC, 2 lymph node metastases from primary OPSCC, 1 oral tongue carcinoma, 3 benign squamous papillomas, and 3 non-oropharyngeal carcinoma tissues).
Representative tracheal and cloacal swabs were chosen to run an EID50 assay for comparison and virus titers were determine by the method of Reed and Meunch [52].
Unligated PlexinC1 and Rap1B mixed at the same concentrations were subjected to the same assay for comparison.
Also, DDsilico outputs the sum of nucleotides within each bin, corresponding to the fluorescence intensity in the Bioanalyzer assay, for comparison with the empirical digestion.
In addition, the CBER reference antigen for H3 A/PertHA16/2009 HA (lot# 70) was also included in the assay for comparison with rHA proteins.
The commercial keratinolytic proteases esperase (5860, Sigma-Aldrich), Alcalase (4860, Sigma-Aldrich), and Savinase (3111, Sigma-Aldrich) were also included in the assay for comparison.
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The activity of these mutant enzymes (Δ239TgAaaH1&2) was assayed for comparison to full length enzymes.
The resulting transmission data are summarized in table 1, together with those from sCJD cases assayed for comparison.
The same tissue RNA pairs and cDNA microarray chips were also used for the regular cDNA microarray assays for comparison.
mRNA prepared from three pairs of hepatoma and non-hepatoma liver tissues was subjected to the SSH/microarray assays, as well as directly to regular cDNA microarray assays for comparison.
By screening known coding-change polymorphisms for function, we were able to perform a directed genetic association study largely focused on this functional variant and including another nearby coding-change variant which lacked functional significance in our assays for comparison.
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CEO of Professional Science Editing for Scientists @ prosciediting.com